糖心vlog

They say a picture is worth a thousand words, but in science, it sometimes takes a thousand photos to tell the whole story.

糖心vlog researchers have assembled the first atomic-level resolution image of part of a molecule that produces force in muscle contraction, an accomplishment that is opening new avenues of research into how muscles contract.

The process to get there involved taking 7,000 photos of the molecule at work in a curious creature called a Thai giant water bug, or Lethocerus indicus.

鈥淭his molecule is designed to be moving or at work, so each image is a little different than the next,鈥� said Hamidreza Rahmani, a physics doctoral student working with Professor of Biological Science Ken Taylor. 鈥淛ust getting to that one image is a challenge.鈥�

The new study is published in the Proceedings of the National Academy of Science.

Scientists have long examined the flight muscles from Lethocerus indicus as a way to better understand how the human heart works. The insect鈥檚 flight muscle and a mammal鈥檚 heart both beat rhythmically.

鈥淚t鈥檚 a strange bug, but it鈥檚 lighting the way to understand muscle contraction,鈥� Taylor said.

Not only can this bug swim and fly, it hunts for fish, frogs, small snakes and even turtles. However, these predatory insects are not without predators themselves as they make very popular snacks or sauce seasoning.

Taylor鈥檚 lab has long been focused on the flight muscles of the giant water bug, lately concentrating on a filament in the muscle that is made of a chain of proteins called myosin, which produces the power needed to contract muscles. Part of the myosin is a molecular motor. The rest of the chain allows the motors to polymerize into filaments so that the motors can act collectively. But these filaments are so small that getting a quality image of them 鈥� and thus a better understanding of how they operate 鈥� has been extremely difficult.

In 2016, Taylor鈥檚 lab published an image of the molecular motor of this protein within its filaments but with insufficient resolution to see individual atoms. Their latest image reveals the individual atoms of the tail. Getting a quality image of the tail was victory enough, but it also revealed unique traits that this motor protein possesses only when assembled into filaments.

Rahmani and Taylor鈥檚 image revealed that the myosin tail had an exceptionally long structural motif called a coiled coil 鈥� alpha helices that are coiled together like a rope. Along this structure, they identified four amino acids known as skip residues. Skip residues typically cause a coiled coil to unwind or even break its alpha-helical structure, but Taylor and Rahmani found that one of these skip residues appeared to have absolutely no effect on the structure, leaving them temporarily baffled.

Taylor began thinking about what this unusual skip residue meant when he remembered that when the muscle is activated 鈥� which allows the heads to produce tension 鈥� the coiled coil lengthens by about 1.5%. He and Rahmani believe that the particular skip residue must be related to that effect.

鈥淭he change occurs just when the muscle is going from relaxed to active but before tension develops,鈥� he said. 鈥淪o, we thought that probably this particular place is very important to that phenomenon.鈥�

Rahmani and Taylor hope to follow up this work with an atomic resolution view of the active filament to gain an understanding of the role skip residues and coiled coil lengthening play in muscle activation.

This work was funded by the National Institutes of General Medical Sciences. Rahmani is also supported by a fellowship from the American Heart Association.

Researchers from University of California, San Diego, Duke University and the Illinois Institute of Technology also contributed to this paper.